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. 2017 Jul 4;8:156. doi: 10.1186/s13287-017-0606-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterization of smooth muscle progenitor cells (pSMCs) derived from male and female hPSCs. a Derived pSMCs exhibited the characteristic “spindle” appearance of SMC phenotype after culture in SMGS medium for 14 days. Cell morphology was unaffected by E2 treatment (0 and 10 nM) for 14 days. Scale bar, 100 μm. b Characterization of pSMCs derived from hPSCs by immunofluorescence staining. More than 95% of derived pSMCs expressed calponin and desmin, >90% SM-22, and >85% SMA when superimposed with DAPI. Blue, DAPI-stained nuclei. Representative image of three independent experiments. Scale bar, 100 μm. SMA smooth muscle actin, DAPI 4,6-diamidino-2-phenylindole, F female, M male (Color figure online)