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. 2017 Jul 4;8:156. doi: 10.1186/s13287-017-0606-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Effect of cell sex on cell proliferation of hPSC-derived smooth muscle progenitor cells (pSMCs). a, b Cell mitosis of male and female pSMCs was monitored with time-lapse dark-field microscopy for 48 h. Graphs show the total (cumulative) number of mitotic events of the male and female pSMCs cocultured with or without E2. c, d Cell mitosis/cell death ratio for hESC and iPSC male/female pairs. ^# p < 0.05 by Student’s t test, male pSMCs vs female pSMCs. *p < 0.05 vs untreated cells; data analyzed by two-way ANOVA followed by Tukey post-hoc test. Data shown represent the mean ± SD from three independent experiments, each performed in triplicate. F female, M male