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. 2017 Jul 4;17:10. doi: 10.1186/s12861-017-0152-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of E-64 (1 μM) during embryo culture medium on a blastocyst total cell number and b percentage of DNA fragmentation assessed by TUNEL assay. Mean values with asterisk denote significant difference at P < 0.05 compared to control. c TUNEL-positive cells that appeared in yellow-green. Scale bar represents 50 μm