Table 1.

Immunization schedule of HIVIS-DNA, HIVISopt-DNA and HIV-MVA-CMDR.

Groups of immunized animals	Day 0	Days 21, 42
1 A. 1 x HIVIS-DNA; 1x MVA	DNA id/EP	1 x MVA im
Content	Env A, B, C/Rev	Env E
Content	Gag p37A B, RT B	Gag Pol A
1 B. 1 x HIVIS-DNA; 2 x MVA	DNA id/EP	2 x MVA im
Content	Env A, B, C/Rev	Env E
Content	Gag p37A B, RT B	Gag Pol A
2 A. 1 x HIVIS-DNAopt; 1 x MVA	DNAopt id/EP	2 x MVA im
Content	Env A, B, C	Env E
Content	Gag p37A B, RTPR B	Gag Pol A
2 B. 1 x HIVIS-DNAopt; 2 x MVA	DNAopt id/EP	2 x MVA im
Content	Env A, B, C	Env E
	Gag p37A B, RTPR B	Gag Pol A
3 A. 1 x MVA	MVA im
Content	Env E
Content	Gag A, Pol A
3 B. 2 x MVA	MVA im	1 x MVA im
Content	Env E	Env E
Content	Gag A, Pol A	Gag A, Pol A
4. Naive controls	none	none

Five Balb/c mice were immunized per group. HIV-DNA doses: 200 μg DNA Gag and RT/PR, 200 μg Env A-C, Rev/mouse/immunization; id Bioject and id EP. 107 pfu of MVA-CMDR/mouse was divided equally between the right and left hind legs, 50 μl/side. Mice were sacrificed at day 30 (groups 1A, 2A, 3 A), or days 63-70 (groups 1B, 2B, 3B).