Table 3.
ELISpot IFNγ cellular responses against peptide pools representing HIV-1 RT and PR of subtype B.
| Responder frequencies with HIV RT and PR peptides |
|||
|---|---|---|---|
| RT I | RT II | PR | |
| (aa 38–109) | (aa 110–179) | (aa 12–38) | |
| Groups of immunized animals | |||
| Group 1A | |||
| 1 x HIVIS; 1 x MVA | 3/5 | 5/5 | 2/5 |
| Median IFNγ SFC/106 | 40 | 45 | 144 |
| Range | (30–73) | (35–95) | (55–152) |
| Group 2A | |||
| 1 x HIVISopt; 1 x MVA | 2/5 | 3/5 | 3/5 |
| Median IFNγ SFC/106 | 33 | 105 | 196 |
| Range | (29–112) | (35–95) | (61–1744) |
| Group 3A | |||
| 2 x MVA | 1/5 | 2/5 | 2/5 |
| Median IFNγ SFC/106 | 96 | 167 | 108 |
| Range | 96 | (40–294) | (98–118) |
Abbreviations: RT I = N-terminal peptides of reverse transcriptase protein subtype B, RT II = C-terminal peptides of reverse transcriptase protein subtype B, PR = peptides representing the protease protein of subtype B.
Group 1A received RT components of subtype A (MVA), B (DNA); no PR.
Group 2A received RT components of subtype A (MVA), B (DNA); PR of subtype B (DNA).
Group 3A received RT components of subtype A (MVA); PR of subtype A (MVA).