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. 2017 Jul 4;19:50. doi: 10.1186/s12968-017-0361-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Injury Patterns on Histology. Images show TTC, Thioflavin S and H&E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H&E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells (open arrow heads) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups (arrows) along with the presence of inflammatory cells (closed arrow heads)