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. 2017 Jul 4;15:49. doi: 10.1186/s12951-017-0284-3

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Phosphofructokinase (PFK) activity (a), mitochondrial membrane potential (ΔΨ_m) (b) and ATP production (c) were assessed in SH-SY5Y cells undifferentiated controls (CO UNDIFF), differentiated controls (CO DIFF) and exposed to PCL-NPs before (NP DIFF) or during (DIFF NP DIFF) differentiation. The histograms represent values calculated as percent of undifferentiated controls. Values are expressed as milliunits/ml/mg protein for PKF activity (a), fluorescence intensity unit (FIU)/mg protein for ΔΨ_m (b), as luminescence/mg protein for ATP production (c). Error bars represent the mean + SEM. Significant differences to undifferentiated and differentiated controls are labeled with asterisks (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ***p ≤ 0.0001)