Table 1.

Enzymatic activities of respiratory chain complexes

Experimental group	Citrate synthase activity (mU/mg protein)	Enzymatic complex activities (mU/mU citrate synthase)
		CI	CII	CIII	CIV	CV
CO DIFF	111 ± 7	0.1 ± 0.01	0.15 ± 0.01	0.13 ± 0.01	0.18 ± 0.01	0.39 ± 0.03
DIFF NP DIFF	131 ± 8	0.07 ± 0.01	0.14 ± 0.01	0.14 ± 0.01	0.13 ± 0.01	0.35 ± 0.03
NP DIFF	127 ± 9	0.08 ± 0.01	0.16 ± 0.02	0.14 ± 0.01	0.15 ± 0.01	0.37 ± 0.05
CO UNDIFF	118 ± 12	0.08 ± 0.01	0.14 ± 0.01	0.18 ± 0.02	0.13 ± 0.03	0.40 ± 0.04

Enzymatic activities of respiratory chain complexes were measured on day in vitro 8 (DIV8) in undifferentiated control cells (CO UNDIFF), differentiated control cells (CO DIFF) and PCL-NPs exposed cells before (NP DIFF) or during (DIFF NP DIFF) differentiation. Values were estimated by the difference in activity levels measured in presence and absence of specific inhibitors; values are expressed as ratios to the mitochondrial marker enzyme citrate synthase (mU/mU citrate synthase) ± S.E.M