Fig. 5.

Results of loss-of-heterozygosity (LOH) analysis. The scheme shows chromosome 9q with a TSC1-associated region and the distribution of microsatellite markers we examined LOH. Genomic DNA was isolated from normal lung tissue, and tumor cells were microdissected from uterine or lung specimens. The pattern of LOH was identical to that at the microsatellite markers D9S2126, D9S1830 and D9S1199, whereas heterozyosity was retained at the remaining microsatellite markers. Arrows indicate the disappearance of alleles.