Table 1.

Epithelial and mesenchymal circulating tumor cell recovery from methods of CTC isolation

Methods	Detection of Epithelial (E) versus Mesenchymal (M) CTCs	References
Size‐based filtration	E & M	Kim et al. (2017), Vona et al. (2000)
Density‐based centrifugation	E & M	Liu et al. (2015)
Size and deformability	M > E	Gogoi et al. (2016)
Cell surface charge	E & M	Gupta et al. (2012)
Negative selection
Microfluidic size based followed by negative selection for CD45	E & M	Karabacak et al. (2014)
Density separation of tetrameric antibody complexes for CD45, CD66b, and glycophorin	E & M	Naume et al. (2004)
Positive selection
Cell surface vimentin	M	Satelli et al. (2015a)
High‐throughput microscopy for immunofluorescence or DNA/RNA FISH	E & M	Krivacic et al. (2004)
EpCAM‐based immunomagnetic separation	E	Allard et al. (2004)
Flow cytometry for surface epithelial markers	E	Gross et al. (1995), Magbanua et al. (2015)
Filtration using selective size amplification	E	Kim et al. (2012)