Fig. 5. In vivo MET activation assays. FVB mice were injected intravenously with PBS (ctrl), 25 pmol K1B (250 ng), 25 pmol K1B/S complex (250 ng K1/700 ng S), 25 pmol NK1 (500 ng) or 2.5 pmol mature HGF/SF (250 ng) per g of body weight. After 10 min, livers were extracted, snap frozen and crushed. MET, Akt and ERK phosphorylation status in cell lysates was analyzed by Western blot. The data shown are representative of three independent experiments which were performed using two mice per condition.
