Figure 11.

The effect of XeC on Ca²⁺ transients in ICC-MY. (A) Representative heat map showing the summated PTCLs of ICC-MY in control and XeC (10 µM). (B) Occurrence map of individually color-coded Ca²⁺ firing sites in the ICC-MY network in control and XeC conditions. (C) Traces of PTCL activity over an entire recording of the ICC-MY network in control conditions and in the presence of XeC (10 µM) showing PTCL area (dark blue) and PTCL count (brown). (D and E) Histogram showing the probability (%) that an individual Ca²⁺ firing site in the ICC-MY cell somata and cell processes in E will fire during a CTC cycle in the presence of XeC (10 µM; red bars) compared with control conditions (black bars; n = 3, FOV = 3). (F) Summary showing that the number of Ca²⁺ firing sites in cell soma was not significantly affected by XeC (10 µM; P = 0.22). (G) PTCL area/frame in the cell somata were 71.42 ± 19.54 µm² in control and 74.36 ± 28.58 µm² in XeC (10 µM; P = 0.86, n = 3, FOV = 3). (H) The PTCL count/frame in the cell soma was 2.71 ± 0.95 in control and 2.69 ± 1.17 in XeC (10 µM; P = 0.95, n = 3, FOV = 3). (I) The number of Ca²⁺ firing sites in the cell processes per FOV changed from 66.3 ± 3.7 in control to 68 ± 22.05 in XeC (10 µM; P = 0.93, n = 3, FOV = 3). (J) PTCL area/frame in the cell processes was 212.4 ± 59.47 µm² in control and 120.2 ± 58.21 µm² in XeC (10 µM; P = 0.12, n = 3, FOV = 3). (K) The PTCL count/frame in the cell processes was 5.27 ± 1.49 in control and 4.98 ± 2.18 in XeC (10 µM; P = 0.95, n = 3, FOV = 3). ns, P > 0.05. Mean ± SE is shown.