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. 2017 Jul 3;149(7):703–725. doi: 10.1085/jgp.201711771

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© 2017 Drumm et al.

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Figure 4. — Quantification of Ca²⁺ transients in ICC-MY. (A) Representative image of an ICC-MY network from the small intestine. Scale bar in A applies to A–D. (B) Full network mask of the ICC-MY network taken from the video shown in A. (C) Cell somata mask of the ICC-MY taken from the video shown in A. (D) Cell processes mask of the cellular processes of the ICC-MY network taken from the video shown in A. (E) Histogram showing the frequencies of CTCs in the ICC-MY network (n = 31, FOV = 56). (F) Histogram showing the probability (%) that an individual Ca²⁺ firing site in the ICC-MY cell somata (black bars) and cell processes (red bars) will fire during a CTC cycle (n = 31, FOV = 56). (G) Histogram showing the range of number of Ca²⁺ firing sites per cell soma in ICC-MY (n = 31, FOV = 56). (H) Histogram showing the range of values for Ca²⁺ transient PTCL area/frame in the ICC-MY cell somata (n = 31, FOV = 56). (I) Histogram showing the range of values for Ca²⁺ transient PTCL count/frame in the ICC-MY cell somata (n = 31, FOV = 56). (J) Histogram showing the range of number of Ca²⁺ firing sites per cell processes FOV (60× magnification) in ICC-MY (n = 31, FOV = 56). (K) Histogram showing the range of values for Ca²⁺ transient PTCL area/frame in the ICC-MY cell processes (n = 31, FOV = 56). (L) Histogram showing the range of values for Ca²⁺ transient PTCL count/frame in the ICC-MY cell processes (n = 31, FOV = 56).