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. 2017 Jul 3;149(7):703–725. doi: 10.1085/jgp.201711771

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© 2017 Drumm et al.

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Figure 7. — The effect of NNC 55-0396 on Ca²⁺ transients in ICC-MY. (A) Representative heat maps showing the summated PTCLs for an entire recording of ICC-MY under control conditions and in NNC 55-0396 (1 µM). (B) Occurrence map of individually color coded Ca²⁺ firing sites in the ICC-MY network in control and NNC 55-0396 (1 µM) conditions. (C) Traces of PTCL activity of the ICC-MY network in control conditions and in the presence of NNC 55-0396 (1 µM) showing PTCL area (dark blue) and PTCL count (brown). (D) Histogram showing the probability (%) that an individual Ca²⁺ firing site in the ICC-MY cell somata will fire during a CTC cycle under control conditions (black bars) and in NNC 55-0396 (1 µM; red bars; n = 6, FOV = 8). (E) Histogram showing the probability (%) that an individual Ca²⁺ firing site in the ICC-MY cell processes will fire during a CTC cycle in control conditions (black bars) and in NNC 55-0396 (1 µM; red bars; n = 6, FOV = 8). (F) The number of Ca²⁺ firing sites in the cell soma was reduced from 4.1 ± 0.5 sites per cell in control to 0.8 ± 0.2 sites per cell in NNC 55-0396 (1 µM; P = 0.0009, n = 6, FOV = 8). (G) PTCL area/frame in the cell somata were reduced from 3.5 ± 1 µm² in control to 0.3 ± 0.25 µm² in NNC 55–0396 (1 µM; P = 0.017, n = 6, FOV = 8). (H) PTCL count/frame was significantly reduced in the cell somata from 0.16 ± 0.04 in control to 0.03 ± 0.02 in NNC 55-0396 (1 µM; P = 0.022, n = 6, FOV = 8). (I) In the cell processes, the number of Ca²⁺ firing sites was reduced from 51 ± 10.7 per FOV in control to 18.5 ± 5.1 per FOV in NNC 55-0396 (1 µM; P = 0.005, n = 6, FOV = 8). (J) In the cell processes, PTCL area/frame was reduced from 18.4 ± 5.9 µm² in control to 1.9 ± 0.8 µm² in NNC 55–0396 (1 µM; P = 0.029, n = 6, FOV = 8). (K) PTCL count/frame was also significantly reduced in the cell processes, changing from 0.7 ± 0.19 in control to 0.1 ± 0.04 in NNC 55-0396 (1 µM; P = 0.014, n = 6, FOV = 8). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Mean ± SE is shown.