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. 2017 Jul 4;10:213. doi: 10.3389/fnmol.2017.00213

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Copyright © 2017 Zhang, Zhang, Yu, Hu, Zhou, Guo, Xu, Zhu, Waqas, Qi, Zhang, Liu, Chen, Tang, Qian, Shi, Gao and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FACS sorting of Lgr5+ cells in the neomycin-treated and untreated cochleae of postnatal mice. (A,B) Neomycin was added for 12 h to the P1 Lgr5-EGFP-creER/Rosa26-tdTomato mouse cochleae, and the same amount of PBS was added to the untreated cochlear culture medium for the same amount of time. After a 24 h recovery period, the cochleae were trypsinized and dissociated into single cells for FACS sorting. (C) PCA analysis for all three replicates of NLPs (Neo1, Neo2, Neo3) and ULPs (Con1, Con2, Con3). (D) Venn diagram showing the number of genes expressed in NLPs (Neomycin) and ULPs (Control).