Table 1.
Olignucleotides used in this study.
| Target | Oligo | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| C. trachomatis omcB | Primer (F)a | GAC ACC AAA GCG AAA GAC AAC AC | 106 |
| Primer (R)a | ACT CAT GAA CCG GAG CAA CCT | ||
| ABI – Probeb | [FAM]-CCA CAG CAA AGA GAC TCC CGT AGA CCG-[QSY] | ||
| Rotor-Gene - Probeb | [FAM]-CCA CAG CAA AGA GAC TCC CGT AGA CCG-[BHQ] | ||
| C. trachomatis pORF2 | Primer (F)a | CAG CTT GTA GTC CTG CTT GAG AGA | 109 |
| Primer (R)a | CAA GAG TAC ATC GGT CAA CGA AGA | ||
| ABI Probeb | [NED]-CGG GCG ATT TGC CTT-[MGBNFQ] | ||
| Rotor-Gene - Probeb | [JOE]-CCC CAC CAT TTT TCC GGA GCG A-[BHQ1] | ||
| H. sapiens RPP30 | Primer (F)a | AGA TTT GGA CCT GCG AGC G | 65 |
| Primer (R)a | GAG CGG CTG TCT CCA CAA GT | ||
| ABI – Probeb | [VIC]-TTC TGA CCT GAA GGC TCT GCG CG-[QSY] | ||
| Rotor-Gene – Probeb | [Cy5]-TTC TGA CCT GAA GGC TCT GCG CG-[BHQ2] |
ABI: Applied Biosystems; Bp: base pairs; F: forward; omcB: outer membrane protein complex B; pORF2: plasmid open reading frame 2; R: reverse; RPP30: RNase P/MRP 30-kDa subunit.
a
Primers purified by desalting.
b
Probes purified by high-performance liquid chromatography.