Table 6.
qPCR assay costs.
| Costing category | Assay platform cost (US$) |
|||
|---|---|---|---|---|
| artus | ABI | ddPCR | Rotor-Gene | |
| DNA extraction (kit & consumables) | 4.70 | 4.70 | 4.70 | 4.70 |
| PCR reaction mix (inclusive of oligos) | 17.18 | 1.70 | 2.84 | 1.10 |
| Lab consumables | 0.41 | 0.12 | 1.84 | 0.35 |
| Personnel monthly salary multiplied by 1.2 for overheads and divided by 160 for hourly rate (based on a trained laboratory technician from Moshi, Tanzania) | 3.37 | 5.06 | 6.74 | 3.37 |
| Total | $25.04 | $11.51 | $15.64 | $9.51 |
NOTE: Purchase equipment costs for an ABI 7900HT, Rotor-Gene Q 5plex (equivalent to the 3000 which is no longer available for purchase) and ddPCR working platform are $62,445, $38,142 and $115,154 respectively.