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. 2017 Jul 3;216(7):2217–2230. doi: 10.1083/jcb.201601109

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© 2017 Stik et al.

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Figure 2. — Internalization of AFT- and BFC-derived EVs. (A) Quantification of AFT and BFC EV internalization by LSK cells analyzed by ImageStreamX (Amnis; n = 3). (B) Representative micrographs of LSK cells. Green dots show PKH67-stained EVs (600× magnification). (C) AFT cell expressing CD63-GFP in close contact with a hematopoietic cell (white arrow; 400× magnification). (D) Coculture of AFT-CD63-GFP with LSK cells. ImageStreamX analysis with GFP and CD45 expression. The hematopoietic cell population is contained within the red-framed area R4. R3 represents CD45⁺ GFP⁺ double-positive cells. Percentages are indicated below the graph. (E) Cell representative of R3. (F) Internalization of AFT EV by CD45⁻, CD45⁺, and LSK cells 24 h after intrafemoral injection. Error bars show SEM. *, P < 0.05.