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. 2017 Jul 3;216(7):2217–2230. doi: 10.1083/jcb.201601109

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© 2017 Stik et al.

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Figure 6. — mRNAs and miRNAs transfer from AFT EVs to LSK cells and apoptotic assay. (A) Expression changes of Peg10 and Bag1 mRNAs and miR-451 and miR-221 miRNAs in LSK cells after contact with AFT EVs. Quantitative PCR analyses were normalized using GAPDH for mRNAs and U6 internal control for miRNAs (n = 3). (B) Measurement of apoptosis by annexin V (AnnV) and 7AAD staining in LSK cells cocultured for 18 h with or without AFT EVs in the absence of cytokines (n = 3). (C and E) Expression of Peg10 and miR-451 in lineage-negative cells transfected with siPeg10 (C) or mimic-451 (E). (D and F) Measurement of apoptosis using annexin V and 7AAD staining in lineage-negative cells transfected with siPeg10 (D) and mimic-451 (F; n = 3). All the cultures were performed in absence of cytokines. Error bars show SEM. *, P < 0.05.