Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 3;216(7):2131–2150. doi: 10.1083/jcb.201611138

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Monis et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Dtnbp1^sdy/sdy and Pldn^pa/pa mice have mildly cystic kidneys. (A) Selected confocal images of 12-mo-old wild-type, Dtnbp1^sdy/sdy, and Pldn^pa/pa mouse kidney sections stained with cilia marker Arl13b antibody (red), collecting duct marker AQP2 antibody (green), and basal body marker γ tubulin antibody (blue). Bars, 25 µm. Insets are 285% enlargements. (B) Quantification of mean collecting duct diameters in three regions of the kidney; (Ba) cortex (wild-type and Dtnbp1^sdy/sdy n = 183 collecting ducts per group, Pldn^pa/pa n = 61 collecting ducts), (Bb) medulla to papilla transition (wild-type and Dtnbp1^sdy/sdy n = 183 collecting ducts per group, Pldn^pa/pa n = 61 collecting ducts), and (Bc) tip of the papilla (wild-type and Dtnbp1^sdy/sdy n = 33 collecting ducts per group, Pldn^pa/pa n = 11 collecting ducts). There is an increase in collecting duct diameter in the Dtnbp1^sdy/sdy and Pldn^pa/pa cortex and medulla-to-papilla transition. Additionally, the Dtnbp1^sdy/sdy papilla tip collecting duct diameter is wider. (C) Quantification of mean kidney cilia length and the percentage of cilia per basal body. (Ca) Wild-type and Dtnbp1^sdy/sdy n = 300 cilia per group, Pldn^pa/pa n = 100 cilia. Cilia in the Dtnbp1^sdy/sdy kidneys are shorter compared with control. (Cb) Wild-type and Dtnbp1^sdy/sd n = 315 basal bodies per group, Pldn^pa/pa n = 105 basal bodies. Percentage cilia per basal body is reduced in the Dtnbp1^sdy/sdy kidneys. (D) Selected confocal images of 12-mo-old wild-type, Dtnbp1^sdy/sdy, and Pldn^pa/pa kidney sections stained with proximal tubule marker Lotus tetragonolobus agglutinin (LTA; green) and nuclei with Dapi (red). Most tubules are proximal, and some are marked with “P.” The few distal convoluted tubules are marked with “D.” Arrows label proximal tubule nuclei, and asterisks indicate red blood cells. Bars, 25 µm. Insets are 172% enlargements. (E) Quantification of mean kidney proximal tubule nuclei circumference, mean number of proximal tubule nuclei, and nuclei number per proximal tubule circumference. Wild-type and Dtnbp1^sdy/sdy n = 225 proximal tubules per group, Pldn^pa/pa n = 75 proximal tubules. (Ea) Dtnbp1^sdy/sdy and Pldn^pa/pa proximal tubules have an increased circumference. (Eb) Dtnbp1^sdy/sdy and Pldn^pa/pa proximal tubules have an increase in the mean number of nuclei. (Ec). Increase in nuclei number per proximal tubule circumference. n = 3 mice per wild-type and Dtnbp1^sdy/sdy groups and n = 1 mouse per Pldn^pa/pa group. Tissue from the wild-type and Dtnbp1^sdy/sdy mice was harvested at precisely 12 mo of age. Two of the three Pldn^pa/pa mice died of a known lung fibrosis phenotype during the aging process. Tissue from the remaining Pldn^pa/pa mouse was harvested at 11 mo and 19 d of age. Error bars are SEM. Data were analyzed using one-way ANOVA and the Bonferroni multiple-comparisons test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.