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. 2017 Jul 3;216(7):1925–1936. doi: 10.1083/jcb.201612126

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Figure 1. — NMIIA and NMIIB regulate the integration of the secretory granules into the APM. (A) Diagram of the experimental set up and model of the integration of the secretory granules into the APM of acinar cells in submandibular SGs upon β-adrenergic stimulation. (B and C) ISMic of the SGs in mT/mGFP (B) and IIA/IIB^fl/fl (C) mice after the expression of Ade-Cre. Low magnification of the Cre-transfected cell expressing mGFP (left, arrowheads; Bars, 20 µm) and time-lapse series of the integration of the secretory granules (right, green arrows; bars, 2 µm) with the APM (red dashed lines) are shown. Time 0 represents the point at which the limiting membranes of the granules were detected. (D–G) Quantitative analysis of the integration of the secretory granules in mT/mGFP (D), IIA/IIB^fl/fl (E), IIA^fl/fl (F), and IIB^fl/fl (G) mice. (Top) The diameters of the granules were measured during the integration and reported as a function of time either in cells expressing (green curves) or not expressing (red curves) Cre. Insets show expansion of the first 15 s of integration. (Bottom) Mean granule diameter (Gran. Diam) ± SD at the onset of integration and mean maximum (Max) diameter measured over the course of the 150-s observation window ±SD in Cre⁺ and Cre⁻ cells. *, P < 0.05; ****, P < 0.0001; ANOVA. N = number of granules in 3 (mT/mGFP), 4 (IIA^fl/fl), and 5 (IIA/IIB^fl/fl and IIB^fl/fl) animals.