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. 2017 Jul 4;10:33. doi: 10.1186/s13072-017-0141-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — DNA accessibility in WT and zld mutants. a Shown is the average FAIRE signal (DNA accessibility) in WT embryos for 2500 enhancer-like loci that are binned based on their ZLD (Y-axis) and GAF (X-axis) ChIP binding. b Same as A, using FAIRE data from maternal zld mutants [20]. Comparison of the two matrices shows that genomic regions with no GAF binding (in WT) are mostly affected by deletion of zld (left hand side of both matrices), while regions bound by GAF (right hand side) still show high DNA accessibility, suggesting that GAF could ac as a pioneer factor in early fly development, together or independently of Zelda