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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Eur J Cell Biol. 2017 Feb 20;96(3):276–288. doi: 10.1016/j.ejcb.2017.02.003

Figure 5. Expression and localization of Ccdc181 protein.

Figure 5

(A) Protein expression of Ccdc181 during postnatal murine testicular development was analyzed by Western blot using testicular protein extracts of 5- to 50-day-old mice. Ccdc181 expression levels sharply increase around day 25 with the onset of spermatid elongation. Staining of α-Tubulin served as a loading control. Sg, spermatogonia; ESc, early spermatocytes; LSc, late spermatocytes; RS, round spermatids; ES, elongating spermatids; Sp, mature spermatozoa. (B) Localization of Ccdc181 was analyzed by immunohistochemical staining of adult mouse testis sections (a). Ccdc181 staining of the microtubular manchette in elongating spermatids (b, arrows) and the sperm tail (c, arrows) was observed. As a control staining without Ccdc181 antibody was performed (d). Scale bars: 50 μm.