Figure 2. UV-visible spectrum of dialyzed PG-M showing the characteristic signal of morphine at 285 nm indicating the presence of the morphine molecule in its active form within the conjugate structure.
Amounts of morphine in all chemical experiments were calculated based on UV-visible quantification using a calibration curve generated from free morphine. The UV spectrum for each sample was acquired using 30 scans per sample for maximized S/N ratio, and represents N = 3 experimental replicates.