Figure 4. Protein domains responsible for the interaction between RIM-BP2 and exophilin-8.
(A) Schematic representation of mouse RIM-BP2 (left), mouse exophilin-8 (right), and their deletion or point mutants. (B–D) HEK293A cells were transfected with plasmids encoding the indicated proteins shown in (A). The immunoprecipitates with anti-FLAG (B, D) or anti-HA (C) antibodies, as well as 1/30 of the original extracts, were immunoblotted with anti-FLAG, anti-GFP, and anti-HA antibodies. (E) MIN6 cells were infected with adenovirus encoding the indicated proteins. The immunoprecipitates with anti-FLAG antibodies, as well as 1/100 of the original extracts, were immunoblotted with anti-FLAG and anti-RIM-BP2 antibodies.