Figure 5. Exophilin-8 mutant deficient in binding to RIM-BP2 fails to cluster RIM-BP2 and insulin granules at cell corners.
(A) INS-1 832/13 cells were coimmunostained with anti-insulin and either anti-exophilin-8 (upper) or anti-RIM-BP2 (lower) antibodies. (B, C) INS-1 832/13 cells were infected with adenovirus encoding MEF-tagged, wild-type (WT) or PA (ABD) mutant exophilin-8. They were coimmunostained with anti-FLAG and anti-insulin antibodies (B) or with anti-myc and anti-RIM-BP2 antibodies (C). Bars, 10 μm.
Figure 5—figure supplement 1. More images of INS-1 832/13 cells and those expressing wild-type or PA (ABD) mutant exophilin-8.
INS-1 832/13 cells and those expressing MEF-tagged, wild-type (WT) or PA (ABD) mutant exophilin-8 were coimmunostained with the indicated antibodies, as shown in Figure 5. Bars, 10 μm.