Author response image 7. Antibody staining in N2 (Wild Type) animals.
(A-B) Day 1 WT animals stained with rabbit anti-LGG-1 (green middle) and either mouse anti-mCherry (A), or mouse anti-GFP (B) (which here functioned as negative controls). Secondary antibody control had no fluorescence in either green or red channels (data not shown). Left column is merged image DIC, anti-LGG-1 and anti-mCherry or anti-GFP, middle is anti-LGG-1 only (green), and right is either anti-mCherry (A) or anti-GFP (B). Data representative of one experiment (N≥5 animals in each condition).