Author response image 3. mCherry and GFP co-localize with endogenous LGG-1 in transgenic animals expressing mCherry::gfp::lgg-1.
(A-B) Immunofluorescence to detect endogenous LGG-1 (green, rabbit anti-LGG-1 antibody made by Abgent) and GFP (A; red, mouse anti-GFP from Santa Cruz Biotechnology) or mCherry (B; red, mouse anti-mCherry from Clonetech) in dissected intestines of wild-type (WT, top, data also in Figure 1—figure supplement 1D, E) or daf-2(e1370) animals (bottom). Data representative of ≥2 experiments (N≥5 animals in each). Scale bars = 20 µM.