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qPCR workflow. All reaction components except for the primers were dispensed to individual microfuge tubes, to which target-specific forward and reverse primer mixes were added. Reaction mixtures were kept on ice until subjected to PCR. Sufficient qPCR premixes were prepared for each of the six targets to run six assays in duplicate. A. Workflow for initial investigation using KAPA master mix. B. Workflow for comparison between KAPA and SensiFast master mixes.
