. 2017 Jun 20;12:10–14. doi: 10.1016/j.bdq.2017.05.002

Table 1.

qPCR assay features. All six assays were designed using Beacon Designer (Premier Biosoft).

Targets		Primers	Size (bp)	Accession no	Start	End	CG	AT	%GC
UBC2	F:	AGGAAAAGTAGTCCCTTCTC	177	NM_021009	295	471	99	78	56%
UBC2	R:	CGAAGATCTGCATTGTCAAG	177	NM_021009	295	471	99	78	56%
IGF1	F:	CCTTTCAAGCCACCCATTGAC	100	NM_000376	1166	1265	62	38	62%
IGF1	R:	AGCAGCGGGTACAAGATAAATATCC	100	NM_000376	1166	1265	62	38	62%
TP53I3	F:	CTGCTGCCGGTTCTGGAC	96	NM_004881	1778	1873	51	45	53%
TP53I3	R:	CAGGACGATCTTGCCTATGTT	96	NM_004881	1778	1873	51	45	53%
GAPDH	F:	GCACAAGAGGAAGAGAGAGACC	84	NM_000875	1089	1172	52	32	62%
GAPDH	R:	AGGGGAGATTCAGTGTGGTG	84	NM_000875	1089	1172	52	32	62%
VDR1	F:	ATCTGCATCGTCTCCCCAGAT	104	NM_001111283	6731	6834	44	60	42%
VDR1	R:	AGCGGATGTACGTCTGCAGTG	104	NM_001111283	6731	6834	44	60	42%
IGF1R	F:	CTCCTGTTTCTCTCCGCCG	78	NM_002046	1225	1302	47	31	60%
IGF1R	R:	ATAGTCGTTGCGGATGTCGAT	78	NM_002046	1225	1302	47	31	60%