Table 2.
Quantification cycles recorded at each of the different qPCR reaction conditions. Assays were run in duplicate. A. Results from assays carried on the Mic cycler. N/A indicates that no Cq was obtained. B. Results from assays carried out in parallel on the CFX Connect cycler.
| (A) | ||||||
|---|---|---|---|---|---|---|
| 95 °C 5” 60 °C 15” | 93 °C 1” 60 °C 5” | 93 °C 1“60 °C 1” | 90 °C 1” 60 °C 15” | 90 °C 1” 60 °C 1” | 88 °C 1” 60 °C 1” | |
| UBC | 27.45 | 27.75 | 33.38 | 26.74 | 32.34 | 30.09 |
| 27.54 | 27.83 | 34.11 | 26.59 | 33.46 | N/A | |
| IGF1 | 27.23 | 27.14 | 27.12 | 30.01 | 26.76 | 27.25 |
| 27.44 | 27.17 | 27.54 | 26.46 | 26.89 | 27.45 | |
| TP53I3 | 26.31 | 26.67 | 27.62 | 25.05 | 26.31 | 26.52 |
| 26.35 | 26.39 | 27.41 | 25.03 | 26.14 | 26.47 | |
| GAPDH | 18.85 | 18.86 | 18.65 | 18.04 | 18.54 | 18.43 |
| 19.00 | 18.84 | 18.28 | 18.03 | 18.50 | 18.32 | |
| VDR | 25.14 | 25.24 | 25.51 | 25.16 | 23.24 | 26.70 |
| 25.05 | 24.55 | 25.64 | 25.09 | 23.41 | 27.16 | |
| IGF1R | 23.28 | 23.11 | 25.76 | 22.42 | 25.60 | 26.17 |
| 23.31 | 23.00 | 25.15 | 22.70 | 25.98 | 30.29 | |
| (B) | ||||||
|---|---|---|---|---|---|---|
| 95 °C 5” 60 °C 15” | 93 °C 1” 60 °C 5” | 93 °C 1“60 °C 1” | 90 °C 1” 60 °C 15” | 90 °C 1” 60 °C 1” | 8 °C 1” 60 °C 1” | |
| UBC | 26.59 | 27.93 | 27.86 | 26.15 | 29.39 | 27.95 |
| 27.10 | 27.70 | 27.42 | 26.43 | 29.04 | 28.06 | |
| IGF1 | 26.70 | 26.62 | 26.93 | 26.46 | 27.73 | 33.70 |
| 26.61 | 26.61 | 26.76 | 26.65 | 27.49 | 34.14 | |
| TP53I3 | 25.07 | 25.65 | 24.74 | 24.40 | 26.66 | 26.54 |
| 25.63 | 25.56 | 24.70 | 24.58 | 26.51 | 26.50 | |
| GAPDH | 18.13 | 18.40 | 18.41 | 17.99 | 19.07 | 18.99 |
| 18.17 | 18.36 | 18.26 | 18.09 | 18.82 | 19.05 | |
| VDR | 24.50 | 25.05 | 24.92 | 24.99 | 26.44 | 29.41 |
| 24.54 | 24.95 | 24.73 | 24.83 | 26.15 | 30.07 | |
| IGF1R | 22.53 | 23.03 | 22.93 | 22.52 | 23.69 | 24.69 |
| 22.53 | 23.01 | 22.87 | 22.52 | 23.45 | 24.91 |