| Chemicals, Peptides, and Recombinant Proteins |
| DMEM/F-12 medium |
Gibco |
11330-032 |
| Sf-900 II SFM medium |
Gibco |
10902-088 |
| Freestyle 293 medium |
Gibco |
12338-018 |
| Lipofectamine 3000 reagent |
Invitrogen |
L3000015 |
| Cellfectin II reagent |
Invitrogen |
10362100 |
| lauryl maltose neopentyl glycol |
Anatrace |
NG310 |
| Cholesteryl hemisuccinate |
Anatrace |
CH210 |
| Digitonin |
Calbiochem |
300410 |
| 3′,5′-cyclic AMP sodium salt |
Sigma-Aldrich |
A6885 |
| Critical Commercial Assays |
| CNBR-activated Sepharose beads |
GE Healthcare |
17-0430-01 |
| Superose 6, 10/300 GL |
GE Healthcare |
17-5172-01 |
| Deposited Data |
| Human HCN1EM density map |
This study |
EMDB: EMD-8511 |
| Human HCN1EM with cAMP density map |
This study |
EMDB: EMD-8512 |
| Human HCN1EM atomic model |
This study |
PDB: 5U6O |
| Human HCN1EM with cAMP atomic model |
This study |
PDB: 5U6P |
| Experimental Models: Cell Lines |
| CHO-K1 |
ATCC |
CRL-9618 |
| Sf9 |
ATCC |
CRL-1711 |
| HEK293S GnTI-
|
ATCC |
CRL-3022 |
| Recombinant DNA |
| Human HCN1EM
|
This study |
N/A |
| Software and Algorithms |
| Seriel EM |
Mastronarde, 2005 |
http://bio3d.colorado.edu/SerialEM |
| Unblur |
Grant and Grigorieff, 2015b |
http://grigoriefflab.janelia.org/unblur |
| Gctf |
Zhang, 2016 |
http://www.mrc-lmb.cam.ac.uk/kzhang/ |
| RELION |
Scheres, 2012 |
http://www2.mrc-lmb.cam.ac.uk/relion |
| Gautomatch |
Kai Zhang |
http://www.mrc-lmb.cam.ac.uk/kzhang/ |
| EMAN2 |
Tang et al., 2007 |
http://blake.bcm.edu/emanwiki/EMAN2 |
| Blocres |
Cardone et al., 2013 |
https://lsbr.niams.nih.gov/bsoft/programs/blocres.html |
| ResMap |
Kucukelbir et al., 2014 |
http://resmap.sourceforge.net/ |
| SWISS-MODEL |
Biasini et al., 2014 |
https://swissmodel.expasy.org/ |
| UCSF Chimera |
Pettersen et al., 2004 |
https://www.cgl.ucsf.edu/chimera |
| PHENIX |
Afonine et al., 2013 |
https://www.phenix-online.org |
| COOT |
Emsley et al., 2010 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| REFMAC |
Brown et al., 2015; Murshudov et al., 2011
|
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/em_fitting/em_fitting.html |
| ProSMART |
Nicholls et al., 2014 |
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/prosmart/prosmart.html |
| MolProbity |
Chen et al., 2010 |
http://molprobity.biochem.duke.edu |
| EMRinger |
Barad et al., 2015 |
http://fraserlab.com/2015/02/18/EMringer/ |
| HOLE |
Smart et al., 1996 |
http://www.smartsci.uk/hole |
| PyMOL |
Schrodinger LLC, 2015 |
http://www.pymol.org |
| Other |
| R1.2/1.3 400 mesh Au holey carbon grids |
Quantifoil |
1210627 |
