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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Clin Cancer Res. 2017 Jan 13;23(13):3442–3452. doi: 10.1158/1078-0432.CCR-16-1815

Figure 3.

Figure 3

PDGFRA expression is significantly upregulated in STXBP4-positive samples from lung SCC Patients. (A) A cluster diagram of relative expression of PDGFRs and VEGFRs by real-time RT-PCR analysis. Tumor specimens were collected from lung SCC patients with surgical resection. A total of 52 high RNA integrity number (RIN > 2.0) STXBP4-positive samples (n=34) and STXBP4-negative samples (n=18), were used for transcriptome profiling by real-time RT-PCR. (B) PDGFRA mRNA was significantly upregulated in STXBP4-positive lung SCC samples. Relative mRNA levels of PDGFRs and VEGFRs of the STXBP4-positive samples (n=35) and STXBP4-negative samples (n=19); *P < 0.05. (C) Scatter plot of relative mRNA expression levels of PDGFRA and STXBP4. (D) STXBP4 or ΔNp63α induces PDGFRA expression in a lung SCC cell line, EBC-1. The cells were retrovirally transduced with empty vector control (Mock), ΔNp63α or STXBP4. The mRNA levels of PDGFRA, STXBP4 or ΔNp63 were determined by real-time RT-PCR. (E) EBC-1 cells transduced as in (D), were subjected to immunoblotting using antibodies for phospho-PDGFRα (p-PDGFRα), PDGFRα, phospho-p38MAPK (p-p38MAPK), p38MAPK, STXBP4, ΔNp63 or β-Actin.