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. Author manuscript; available in PMC: 2017 Jul 5.
Published in final edited form as: Mucosal Immunol. 2017 Jan 4;10(5):1259–1269. doi: 10.1038/mi.2016.118

Figure 4. Detection of cervix-derived HSV-2 reactive CD8+ T cells using autologous HSV-2 infected DC.

Figure 4

Cells isolated from cervical biopsy samples were co-cultured with autologous mock infected or HSV-2 infected DC and tested ex vivo for the expression of IFN-γ and CD103. (A) Representative dotplots for the expression of IFN-γ and CD103 by CD8+ T cells isolated from a cervical biopsy in response to mock DC (bottom graphs) or HSV-2 DC (bottom graphs). T cells were isolated from a cervical biopsy from a representative HSV-2 infected participant and co-cultured with autologous mock DC or HSV-2 DC and the resultant CD8+ T cells were analyzed by ICS and flow cytometry for the expression of IFN-γ and CD103. (B) Net % HSV-2 reactive CD8+ T cells (CD8+/IFN-γ+) was measured in biopsy samples from 5 HSV-2 infected subjects; the bar depicts the median of all cervical biopsy samples. (C) Expression of CD103 on HSV-2 reactive CD8+ T cells derived from cervical biopsies. The percentage contribution of IFN-γ+ (HSV-2 reactive) cells to the total CD103(+) and CD103(-) CD8+ T cell subsets from 5 participants is displayed. Bars represent the medians.