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. 2017 Jul 4;7:4558. doi: 10.1038/s41598-017-04468-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time course assessment of STBEV uptake. HCAEC uptake of PKH-labelled STBEVs was visualised using fluorescent microscopy (a). A representative image of HCAECs following 6 hours of incubation with PKH-labelled STBEVs. The cell nuclei are labelled blue and PKH-labelled STBEVs are seen as green. Time-dependent uptake of normal (b, N1-N3) and PE (c, PE1-PE3) STBEVs was quantified using flow cytometry. Although variable between samples, all samples showed a time-dependent uptake. The horizontal axis shows mean fluorescent values; a.u., arbitrary units.