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. 2017 Jul 4;7:4565. doi: 10.1038/s41598-017-04786-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antisense transcription detected on the opposite strand of some newly identified RNAs through MiSeq RNA sequencing. RT-PCR was performed on RNA extracted from cells collected at an OD_600nm of 6. Lanes 1 to 5 show IGR_1080208, IGR_1141547, IGR_1141775IGR, IGR_2019414 and 2019159, from left to right after cDNA synthesis (+) or after DNase treatment (−). The gel presented here was cropped for clarity purpose.