Table 6.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Characteristics | Reference | Accession number |
|---|---|---|---|
| S. aureus strains | |||
| Newman | Methicillin-sensible S. aureus strain | 23 | AP009351 |
| N315 | Methicillin-resistant S. aureus strain | 62 | BA000018 |
| HG003 | Derivative strain of NCTC8325 | 63 | JPPU00000000 |
| USA300 FPR3757 | Community-acquired methicillin-resistant clone | 38 | CP000255 |
| UAMS-1 | Oxacillin-susceptible clinical isolate | 64 | JTK00000000 |
| Newman-217 | Newman Δrny::ermC | 35 | |
| Newman-217 pCG296 | Newman Δrny::ermC with pCG296 for complementation | 35 | |
| Newman Δsrn_3610_sprC | Newman strain deleted for sRNA Srn_3610_SprC | 25 | |
| RN4220 | Type I restriction-modification-deficient strain | 65 | |
| E. coli strain | Srn_9343 under its promoter (100 nt) and with 3′ 3xFLAG | This study | |
| XL1 Blue | |||
| Plasmids | |||
| pYAR139 | |||
| pYAR140 | Srn_9343 under its promoter (250 nt) and with 3′ 3xFLAG | This study | |
| pYAR144 | Srn_9343 under its promoter (42 nt) and with 3′ 3xFLAG | This study | |
| pYAR146 | Srn_9343 under its promoter (70 nt) and with 3′ 3xFLAG | This study | |
| pYAR148 | Srn_9343 under Pveg promoter and with 3′ 3xFLAG | This study | |