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. 2017 Jul 4;8:55. doi: 10.1038/s41467-017-00094-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Characteristics of pyrite and spatial pyrite-hypha relation. (a) Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM) volumetric rendering of zeolites, calcite and hyphae (blue, transparent) and pyrite (green, opaque). (b–e) Back-scattered Environmental Scanning Electron Microscopy (ESEM)-images in situ on the hyphal surface, (b) Fine-grained pyrite with hyphae (c) Two parallel hyphae of which the left one is older and has pyrite girdling around the outermost part due to SRB-related precipitation (white arrow), whereas the growth of the right hypha has been influenced by the already present pyrite crystals (white arrow highlights hypha by-passing pyrite). (d, e) Spatial relation of contemporaneous pyrite and hypha, showing that the pyrite has grown on the hypha, yet is in parts enclosed by it (arrows). The features in b–e indicate that SRB-related precipitation of pyrite was contemporaneous with fungal growth. Scale bars (a) 300 µm, (b, c) 10 µm, (d) 25 µm, (e) 50 µm