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. 2017 Jul 4;7:4560. doi: 10.1038/s41598-017-04474-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dietary EPA increases the number of adipose tissue Tregs, eosinophils and B cells in normal chow-fed mice. C57BL/6J mice were fed normal diet free of fish oils or 5% EPA-containing diet for 4–5 weeks. (A) Body weight of C57BL/6J mice during treatment with 5% EPA starting from 5 weeks of age (n = 6). (B) Organ weight of C57BL/6J mice treated with 5% EPA (n = 6). (C) Serum adiponectin levels of mice fasted for 4 hours (n = 6). (D–H) Flow cytometric analysis of SVF of adipose tissues from 5% EPA-treated mice was performed. (D) Number of epididymal adipose tissue Treg per 1 g adipose tissue. (n = 6). (E) Amount of total cell number per g adipose tissue (area of circular chart) and composition of stromal vascular fraction from epididymal adipose tissue (% of total SVF). (F) Total cell number of SVF per adipose tissue (n = 8). (G) Number of preadipocytes, hematopoietic cells and endothelial cells (n = 6). (H) Amount of CD45⁺ immune cell number per g adipose tissue (area of circular chart) and composition of immune cells (% of CD45⁺ cells). (I) Number of adipose immune cells (n = 6). Data are mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001. Epi, epididymal adipose tissue: Sub, subcutaneous adipose tissue: Mesen, mesenteric adipose tissue.