Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 4;7:4560. doi: 10.1038/s41598-017-04474-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Dietary EPA increases the number and proportion of Tregs irrespective of the size of adipose tissue in ob/ob mice. ob/ob mice that lack leptin and express excess food intake and obesity were fed normal diet free of fish oils, or 5% EPA-containing diet for 4–5 weeks. (A) Body weight of ob/ob mice during treatment with 5% EPA starting from 5 weeks of age (n = 8). (B) Organ weight of ob/ob mice treated with 5% EPA (n = 8). (C) Serum adiponectin of mice fasted for 4 hours (n = 8). (D–H) Flow cytometric analysis of SVF of adipose tissues from 5% EPA-treated mice. (D) Number of Epididymal adipose tissue Treg per 1 g adipose tissue (n = 6). (E) Amount of total cell number per g adipose tissue (area of circular chart) and composition of stromal vascular fraction (% of total SVF). (F) Total cell number of SVF per tissue (n = 8). (G) Number of preadipocytes, hematopoietic cell and endothelial cells (n = 8). (H) Amount of CD45⁺ immune cell number per g adipose tissue (area of circular chart) and composition of immune cells (% of CD45⁺ cells). (I) Number of adipose immune cells (n = 8). Data are mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001. Epi, epididymal adipose tissue: Sub, subcutaneous adipose tissue: Mesen, mesenteric adipose tissue.