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. 2017 Jul 4;7:4579. doi: 10.1038/s41598-017-04587-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SAXS analysis for CEH-14–LIM-7. (A) Buffer-corrected, desmeared SAXS scattering curve of CEH-14–LIM-7 (circles; 5.2 mg mL⁻¹) in 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM TCEP, overlaid with the theoretical scattering profiles of an homology model of CEH-14–LIM-7 based on the template of the LHX3-ISL1 crystal structure (1RGT Chain B; cyan line) calculated by CRYSOL. (B) Guinier plot showing linearity of low Q data. (C) The pairwise distribution P(r) profile. (D) Shape restoration model of CEH-14–LIM-7 (white surface) superimposed with an homology model based on the template of the LHX3-ISL1 crystal structure (orange and purple; zinc ions are shown as grey spheres).