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. 2017 Jul 4;7:4586. doi: 10.1038/s41598-017-04771-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MPM images and the corresponding H&E-stained images of ductal adenocarcinoma in the pancreatic head. Magnification of H&E-stained image is 40 ×; Scale: 100 μm. (a) TPEF image; (b) SHG image; (c) Overlay of TPEF and SHG image; (d) H&E-stained image. Position 1: well differentiated neoplastic gland; position 2: poorly differentiated neoplastic glands; position 3: collagen detected by TPEF signal; position 4: collagen detected by SHG signal.