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. 2017 Jul 5;8:1253. doi: 10.3389/fmicb.2017.01253

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Copyright © 2017 Shalev, Turgeman-Grott, Tamir, Eichler and Gophna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cryo-transmission electron microscopy of right-side-out membrane vesicles. The S-layer is compromised in RSO membrane vesicles prepared from H. volcanii ΔaglBΔagl15. (A,B) Electron micrograph of a representative RSO membrane vesicles derived from H. volcanii cells of the WT glycosylation strain (A) and the ΔaglBΔagl15 strain (B). In each panel, the bar represents 100 nm. (C) The percentages of intact S-layer in RSO membrane vesicles prepared from cells of the WT glycosylation strain (left, full symbols; n = 26) and the ΔaglBΔagl15 strain (right, open symbols; n = 62). In each column, the average (horizontal line) and the distribution of values collected from each vesicle population are presented.