Skip to main content
. 2017 Jul 5;7:307. doi: 10.3389/fcimb.2017.00307

Figure 3.

Figure 3

Evidence of TBF in lambs experimentally infected with A. phagocytophilum sheep isolate. Groups of three lambs each were immunized with recombinant MSP4, MSP4-HSP70 combination or adjuvant/saline control and experimentally infected with a sheep isolate of A. phagocytophilum. (A) Rectal temperatures were taken daily until 10 dpi and then weekly until 94 dpi. (B–D) Whole blood was collected in EDTA-containing tubes from the jugular vein of each lamb at different time points for different hematological analyses including (B) hemoglobin, and percent (C) neutrophils and (D) monocytes for leukocyte cell differentiation using an electronic counter (Hemavet 950, Drew, USA). Results from rectal temperature and hematological analyses were compared between immunized and control groups by two-way ANOVA test (*P < 0.05; N = 3 replicates per treatment). Red and blue asterisks denote statistical significant differences between MSP4-HSP70 and MSP4 immunized animals and controls, respectively.