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. 2017 Jul 5;11:189. doi: 10.3389/fncel.2017.00189

Figure 2.

Figure 2

Secretion of IL-1β by U937 cells in the presence of nicotinic acetylcholine receptor (nAChR) antagonists and ACh, Nic or PC. U937 cells were primed with lipopolysaccharide (LPS; 1 μg/ml) for 5 h and BzATP (100 μM) was given for additional 30 min, in the presence or absence of ACh (10 μM) (A), Nic (100 μM) (B) or PC (100 μM) (C). BzATP induced the release of IL-1β, which was inhibited in the presence of ACh, Nic or PC (A–C). The inhibitory potential of ACh (A) was abolished by mecamylamine (Mec, 100 μM), α-bungarotoxin (α-Bun, 1 μM), strychnine (Stry, 10 μM), ArIB [V11L, V16D] (500 nM) or RgIA4 (200 nM). Addition of ArIB or RgIA4 antagonized the effects of Nic (B). Similarly the effect of PC was blunted by Mec, α-Bun, Stry and ArIB (C). The IL-1β concentration was quantified by ELISA. Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney rank sum test and presented as individual data points, bars represent median, whiskers percentiles 25 and 75. *p ≤ 0.05 significantly different from samples where BzATP was given alone.