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. 2017 Jul 5;11:189. doi: 10.3389/fncel.2017.00189

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Copyright © 2017 Zakrzewicz, Richter, Agné, Wilker, Siebers, Fink, Krasteva-Christ, Althaus, Padberg, Hone, McIntosh and Grau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduction of mRNA expression of nAChR subunits α9 and α10 upon siRNA transfection. U937 cells were transfected with scrambled siRNA or with siRNA specific for CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10). Forty-eight hours after transfection, the mRNA expression of α9 (A,B) or α10 (C,D) was analyzed by real-time RT-PCR. Transfection efficiently (A,C) and selectively (B,D) down-regulated the mRNA expression of the gene targeted with gene-specific siRNA. Data were analyzed by Mann-Whitney rank sum test and presented as individual data points, bars represent median, whiskers percentiles 25 and 75. *p ≤ 0.05 significantly different from samples transfected with scrambled siRNA.