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. Author manuscript; available in PMC: 2017 Jul 5.
Published in final edited form as: Nat Struct Mol Biol. 2016 Oct 31;23(12):1124–1131. doi: 10.1038/nsmb.3316

Fig. 3.

Fig. 3

B. cereus fluoride riboswitch cotranscriptional SHAPE-Seq data. (a) The antiterminated and terminated folds26 of the fluoride riboswitch, colored by the reactivity values at lengths 90 nt and 82 nt, respectively (bottom). (b) Cotranscriptional SHAPE-Seq reactivity matrices for the fluoride riboswitch transcribed with 10 mM (top) or 0 mM NaF (bottom). (c) Reactivity differences (Δρ) between the matrices in (b) annotated according to folding events during transcription. The reactivity changes that occur over the course of transcription suggest that the fluoride riboswitch traverses two cotranscriptional folding pathways depending on the presence of ligand. Results shown in b and c are n=1 and are representative of three biological replicates (Supplementary Fig. 9c,d).