1.

Clinicopathological data of all patients and number of captured CTCs

Case	Gender	Age (year)	Tumor site	TNM stage	Pre-treatment capture (n)	Treatment method	Post-treatment capture (n)
1	Male	52	Larynx	IVa	11	Surgery	0
2	Male	62	Hypopharynx	IVa	10	–	–
3	Male	66	Larynx	IVa	17	Surgery	1
4	Male	67	Larynx	I	1	Surgery	–
5	Male	56	Larynx	II	2	Surgery	7
6	Male	59	Larynx	II	0	Surgery	0
7	Male	48	Larynx	II	0	Surgery	–
8	Male	58	Hypopharynx	IVa	0	Induction chemotherapy	–
9	Male	53	Larynx	IVa	3	Surgery	0
10	Male	81	Larynx	III	1	Surgery	0
11	Male	47	Hypopharynx	IVa	5	Induction chemotherapy+Surgery	29
12	Male	65	Hypopharynx	IVa	0	Induction chemotherapy+Surgery	6
13	Male	69	Larynx	IVb	0	Surgery	2
14	Male	74	Larynx	III	5	Surgery	2
15	Male	82	Hypopharynx	III	4	Induction chemotherapy	–
16	Male	70	Larynx	IVa	2	Surgery	0
17	Male	69	Larynx	III	2	Surgery	0
18	Male	50	Hypopharynx	IVa	3	Surgery	0
19	Female	70	Hypopharynx	II	0	Surgery	0
20	Male	66	Hypopharynx	IVa	4	Induction chemotherapy	–
21	Male	73	Hypopharynx	IVa	2	Surgery	4
22	Male	50	Larynx	IVa	2	Surgery	–