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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Nature. 2017 Mar 1;543(7644):270–274. doi: 10.1038/nature21688

Extended Data Figure 3.

Extended Data Figure 3

(a) Chemical structure of dTAG-7.

(b) Dose-responsive FKBP12F36V-ENL degradation detected by immunoblot after 16 hour treatment of MV4;11 (Cas9+, FKBP12F36V-HA-ENL+) cells with DMSO (labelled with “− “) or dTAG-7 at the indicated concentration.

(c) As in (b), but with dTAG-13.

(d) Immunoblot detection of ENL-FKBP12F36V degradation in MV4;11 (Cas9+, ENL-FKBP12F36V-HA+) after 16 hours.

(e) Kinetic evaluation of FKBP12F36V-ENL degradation by dTAG-7 (500 nM) in MV4;11 (Cas9+, FKBP12F36V-HA-ENL+) cells.

(f) Degradation of ENL-FKBP12F36V in MOLM-13 cells following a 4-hour treatment of ENL-FKBP12F36V-expressing cells with dTAG-13 (500 nM).

(g) As in (f), but with NOMO-1 cells expressing ENL-FKBP12F36V.

(h) As in (f), but with JURKAT cells expressing ENL-FKBP12F36V.

(i) Long-term kinetic evaluation of ENL-FKBP12F36V degradation by a single dose of dTAG-7 (500 nM) in MV4;11 (Cas9+, ENL-FKBP12F36V-HA+) cells.

(j) As in (i), but with dTAG-13 (500 nM).