Fig. 1. Identification of peroxiredoxin I as a direct binding protein of triptolide. (a) A silver-stained gel of pull-down products of Biotin-TL or biotin. MDCK cell lysate was incubated with 5 μM biotin or Biotin-TL. Proteins were then purified using streptactin-conjugated agarose beads, followed by SDS-PAGE analysis. The arrow indicates a band confirmed as Prx I. (b) In-gel fluorescence analysis of recombinant Prx I after incubation with Cy3-TL in the absence or presence of TL pretreatment.
